A method of manufacturing the antibacterial modified chitosan-based hemostatic material comprising: (i) preparing materials including: a modified chitosan ingredient and third different solutions including a solution containing polysaccharide, a solution containing dissolving solvent, and a solution containing nano silver; (ii) mixing the solutions prepared in step (i) to create a foundation mixture; (iii) loading the foundation mixture into a electrospinning device according to predetermined specifications to obtain the antibacterial modified chitosan-based hemostatic material in the form of nanofibers; and (iv) applying the antibacterial modified chitosan-based hemostatic material to a bleeding tissue on the surface of skin of a subject in need thereof to prevent bleeding and fluid exudation from the tissue, and promoting bacteriostatic and anti-inflammatory effects on the wound surface.
