A method for purifying exosomes from a cell culture medium comprising combining at least three (03) treating stages of a sample containing exosomes in the following specific order: (A) filtering the sample containing exosomes by tangential flow filtration (TFF) to obtain a TFF-filtered sample; (B) centrifuging the TFF-filtered sample by sucrose cushion centrifugation (SCC) to obtain a SCC-centrifuged sample; and (C) purifying the SCC-centrifuged sample by bind-elute size exclusion chromatography (BE-SEC) to obtain a purified exosomes. The present invention provides a comprehensive and efficient approach for purifying exosomes from a cell culture medium, facilitating using exosomes in various biomedical applications.
