A composition for prevention and treatment of Early Mortality Syndrome (EMS) shrimp to replace antibiotics obtain from process creating a basic mixture by mixing a complex composition with mixing ingredients, then stirring the basic mixture for 1 hour, and adjusting the pH to 6.5-7.5 and leaving it for one day at a temperature of 30° C.-35° C.; wherein the complex composition including 10%-15% by weight of a turkey tail mushroom extract and 10%-15% by weight of a chitosan; in which mixing ingredients consisting of a carboxymethyl cellulose solution 0.375%, a alginate solution 0.375%, a potassium citrate solution, a AgNO3 solution 0.37%, a Cu(NO3)2 solution 0.723%, a Zn(NO3)2 solution 3.085%, Co(NO3)2 solution 0.746%, a Fe(NO3)3 solution 2.5%, a glycerin ingredient, a vitamin E, a tartrazine ingredient 1%, a K2HPO4 solution 15%, a mixture extracts/essential oils, and an other ingredient.
